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Nat Methods. 2008 Nov;5(11):947-9. doi: 10.1038/nmeth.1258. Epub 2008 Sep 15.

Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP.

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  • 1Department of Chemistry, Stanford University, 375 North-South Mall, Stanford, California 94305, USA.

Abstract

The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.

PMID:
18794860
PMCID:
PMC2655310
DOI:
10.1038/nmeth.1258
[PubMed - indexed for MEDLINE]
Free PMC Article
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