Format

Send to

Choose Destination
Microsc Microanal. 2008 Oct;14(5):375-9. doi: 10.1017/S1431927608080781.

An improved cryogen for plunge freezing.

Author information

1
California Institute of Technology, Broad Center, MC 114-96, 1201 E. California Blvd., Pasadena, CA 91125, USA. tivol@caltech.edu

Abstract

The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.

PMID:
18793481
PMCID:
PMC3058946
DOI:
10.1017/S1431927608080781
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Cambridge University Press Icon for PubMed Central
Loading ...
Support Center