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Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14820-5. doi: 10.1073/pnas.0802822105. Epub 2008 Sep 11.

Rifamycins do not function by allosteric modulation of binding of Mg2+ to the RNA polymerase active center.

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1
Laboratory of Molecular Biophysics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.

Abstract

Rifamycin antibacterial agents inhibit bacterial RNA polymerase (RNAP) by binding to a site adjacent to the RNAP active center and preventing synthesis of RNA products >2-3 nt in length. Recently, Artsimovitch et al. [(2005) Cell 122:351-363] proposed that rifamycins function by allosteric modulation of binding of Mg(2+) to the RNAP active center and presented three lines of biochemical evidence consistent with this proposal. Here, we show that rifamycins do not affect the affinity of binding of Mg(2+) to the RNAP active center, and we reassess the three lines of biochemical evidence, obtaining results not supportive of the proposal. We conclude that rifamycins do not function by allosteric modulation of binding of Mg(2+) to the RNAP active center.

PMID:
18787125
PMCID:
PMC2567451
DOI:
10.1073/pnas.0802822105
[Indexed for MEDLINE]
Free PMC Article
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