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Nucleic Acids Symp Ser (Oxf). 2008;(52):281-2. doi: 10.1093/nass/nrn142.

Synthesis of 3'-deoxyapionucleoside triphosphates and their incorporation into DNA by DNA polymerase.

Author information

1
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.

Abstract

Here, we report the synthesis of 3'-deoxyapionucleoside 3''-triphosphates (apioNTPs) and the analysis of their property as substrate of enzymatic polymerization. We established the large scale synthetic route of 3-deoxy-D-apiose from D-galactose, and regio- and stereo-selective glycosylation procedures. Resulting 3'-deoxyapionucleosides were then converted into their 3''-triphosphates. We carried out primer-extension reactions and found that Therminator polymerase, a variant of 9 degrees N DNA polymerase, was an efficient enzyme for incorporation of apioNTPs to synthesize DNA-dependent 3'-deoxy-D-apiose nucleic acids (apioNAs).

PMID:
18776363
DOI:
10.1093/nass/nrn142
[Indexed for MEDLINE]

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