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Am J Med Genet. 1991 Jun 15;39(4):472-3.

Sex identification by polymerase chain reaction using X-Y homologous primer.

Author information

1
Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo, Japan.

Abstract

A method of sex identification using the polymerase chain reaction technique is described. Using a pair of nucleotide primers from an X-Y homologous region, both the X and the Y sequences can be amplified simultaneously, and more importantly, they result in fragments of different lengths. The success of the procedure is therefore monitored by the presence of a X-specific band while sex is identified by the presence or absence of a Y-specific band.

PMID:
1877627
DOI:
10.1002/ajmg.1320390420
[Indexed for MEDLINE]

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