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Trends Plant Sci. 2008 Oct;13(10):517-20. doi: 10.1016/j.tplants.2008.08.002. Epub 2008 Sep 2.

Boosting tandem affinity purification of plant protein complexes.

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1
Department of Plant Systems Biology, Flanders Institute for Biotechnology and Department of Molecular Genetics, Ghent University, Technologiepark 927, 9052 Gent, Belgium.

Abstract

Protein-interaction mapping based on the tandem affinity purification (TAP) approach has been successfully established for several systems, such as yeast and mammalian cells. However, relatively few protein complex purifications have been reported for plants. Here, we highlight solutions for the pitfalls and propose a major breakthrough in the quest for a better TAP tag in plants.

PMID:
18771946
DOI:
10.1016/j.tplants.2008.08.002
[Indexed for MEDLINE]
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