Format

Send to

Choose Destination
Curr Protoc Cytom. 2003 May;Chapter 6:Unit 6.15. doi: 10.1002/0471142956.cy0615s24.

Whole blood analysis of leukocyte-platelet aggregates.

Author information

1
University of Massachusetts Medical School, Worcester, Massachusetts, USA.

Abstract

In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils. The platelet binding is initiated primarily through platelet surface expression of P-selectin (CD62P) following activation-dependent degranulation. The levels of P-selectin involved can be low enough to make direct measurement difficult, but detection of leukocyte-platelet aggregates is relatively simply by whole-blood flow cytometry. Light scatter and at least one leukocyte-specific antibody are used to gate the desired population, and the presence of associated platelets is detected by immunostaining for abundant platelet-specific markers.

PMID:
18770779
DOI:
10.1002/0471142956.cy0615s24
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center