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Curr Protoc Stem Cell Biol. 2008 Jul;Chapter 1:Unit 1F.4. doi: 10.1002/9780470151808.sc01f04s6.

Differentiation of mouse embryonic stem cells into blood.

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  • 1Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, USA.

Abstract

Embryonic stem (ES) cells can be maintained as pluripotent stem cells or induced to differentiate into many different somatic cell types. As ES-derived somatic cells can potentially be used for cell transplantation or cell-based therapy, ES cells have gained much scientific and general public attention. Successful derivation of blood from ES cells for tissue engineering will require a comprehensive understanding of inductive signals and downstream effectors involved in blood lineage development. Ideally, directed differentiation of ES cells into blood and isolation of pure hematopoietic progenitors will enhance our ability to utilize ES-derived blood cells for future clinical applications. The protocols provided in this unit describe methods of maintaining and differentiating mouse ES cells as well as identifying and isolating hematopoietic progenitors by utilizing flow cytometry and progenitor assays.

PMID:
18770633
PMCID:
PMC2640320
DOI:
10.1002/9780470151808.sc01f04s6
[PubMed - indexed for MEDLINE]
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