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Curr Protoc Microbiol. 2007 Jun;Chapter 12:Unit 12E.4. doi: 10.1002/9780471729259.mc12e04s05.

Genetic manipulation of Leptospira biflexa.

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Unité de Biologie des Spirochètes Institut Pasteur, Paris, France.


The genus Leptospira belongs to the order Spirochaetales and is composed of both saprophytic and pathogenic members, such as Leptospira biflexa and L. interrogans, respectively. A major factor contributing to our ignorance of spirochetal biology has been the lack of methods available for genetic analysis of these organisms. In recent years, an E. coli-L. biflexa shuttle vector has been constructed and a system for targeted mutagenesis and random transposon mutagenesis of the saprophyte L. biflexa has been developed. These studies enable the use of L. biflexa as a model bacterium among spirochetes.

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