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J Biomed Biotechnol. 2008;2008:394802. doi: 10.1155/2008/394802.

Jaceosidin induces apoptosis in human ovary cancer cells through mitochondrial pathway.

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1
The Second Affiliated Hospital, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.

Abstract

We examined the antiproliferation effect of Jaceosidin (4', 5, 7-trihydroxy-3', 6-dimethoxyflavone) isolated from the herb of Artemisia vestita Wall on several human cancer cell lines. Jaceosidin significantly reduced the proliferation of CAOV-3, SKOV-3, HeLa, and PC3 cells in a concentration-dependent manner. A time-dependent inhibition was also observed in CAOV-3 cells by Jaceosidin. By flow cytometric analysis, we found that Jaceosidin treatment resulted in an increased apoptosis in CAOV-3 cells. The cells treated with Jaceosidin exhibited a decreased mitochondrial membrane potential. Jaceosidin also increased the level of cleaved caspase-9 and induced the cleavage of caspase-3 and poly (ADP-ribose) polymerase (PARP), while caspase-3 inhibitor Z-DEVD-FMK significantly reversed the proapoptotic effect of Jaceosidin in CAOV-3 cells. Moreover, Jaceosidin elevated the level of cytochrome c in cytosol. These findings suggest that the anticancer effect of Jaceosidin may be contributed by an induction of apoptosis involving cytochrome c release from mitochondria to cytosol.

PMID:
18769496
PMCID:
PMC2519793
DOI:
10.1155/2008/394802
[Indexed for MEDLINE]
Free PMC Article
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