Abstract
The beta-endorphin 10(-7-)-10(-11) M in LPS (lypopolisaccharide) presence and in spontaneous cultures promoted the IL-1beta production in mixed leukocyte fraction. LPS-induced IL-8 production in leukocyte fraction was inhibited by beta-endorphin 10(-7), 10(-11) M. The enchasing effect of beta-endorphin on IL-1beta production was not blocked by naloxone and naltrindole. The inhibitory effect of beta-endorphin on IL-8 production was blocked by naloxone and naltrindole. In mononuclear and neutrophile fractions beta-endorphin and delta-agonist DADLE enchased IL-1beta production in spontaneous and LPS-stimulating cultures, when IL-8 production inhibited beta-endorphin and delta-agonist DADLE only in LPS presence. No effect of mu-agonist DAGO were observed on IL-1beta production, whereas LPS-induced IL-8 secretion in neutrophile fraction inhibited by DAGO.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Cells, Cultured
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / pharmacology
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Enkephalin, Leucine-2-Alanine / pharmacology
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Humans
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Interleukin-1beta / biosynthesis*
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Interleukin-8 / biosynthesis*
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Leukocytes / drug effects
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Leukocytes / metabolism*
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Leukocytes, Mononuclear / drug effects
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Leukocytes, Mononuclear / metabolism
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Lipopolysaccharides / pharmacology
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Male
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Neutrophils / drug effects
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Neutrophils / metabolism
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Oligopeptides / pharmacology
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Receptors, Opioid, delta / agonists*
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Receptors, Opioid, delta / antagonists & inhibitors
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Receptors, Opioid, mu / agonists*
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Receptors, Opioid, mu / antagonists & inhibitors
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beta-Endorphin / pharmacology
Substances
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Interleukin-1beta
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Interleukin-8
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Lipopolysaccharides
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Oligopeptides
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Receptors, Opioid, delta
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Receptors, Opioid, mu
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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deltorphin II, Ala(2)-
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beta-Endorphin
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Enkephalin, Leucine-2-Alanine