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J Cancer Res Clin Oncol. 2009 Feb;135(2):173-9. doi: 10.1007/s00432-008-0461-8. Epub 2008 Sep 3.

Curcumin diminishes human osteoclastogenesis by inhibition of the signalosome-associated I kappaB kinase.

Author information

1
Department of Hematology and Oncology, Charitéplatz 1, Charité, Universitätsmedizin Berlin, 10117, Berlin, Germany.

Abstract

PURPOSE:

Curcumin is a natural polyphenolic derogate extracted from spice turmeric, exhibiting anti-inflammatory and chemopreventive activities. It was described to interact with the signalosome-associated kinases and the proteasome-ubiquitin system, which both are involved in the osteoclastogenesis. Thus, we hypothesized that curcumin could diminish osteoclast differentiation and function.

METHODS:

For the experiments considering osteoclast differentiation and resorptional activities, preosteoclasts were cultured for 4 weeks and treated with curcumin at subapoptotic dosages. Derived mature osteoclasts were identified as large, multinucleated cells with expression of tartrate-resistant acid phosphatase activity. Formation of resorption lacunae, a hallmark of osteoclast activity, was quantified using dentine pits and light microscopy. The signaling pathways were examined by ELISA-based methods and by immunoblotting.

RESULTS:

Both 1 and 10 microM curcumin abrogated osteoclast differentiation (by 56 and 81%) and function (by 56 and 99%) (P < 0.05) dose-dependently. The effects were accompanied by the inhibition of I kappaB phosphorylation and NF-kappaB activation. In contrast, subtoxic doses did not have any significant effects on proteasome inhibition.

CONCLUSION:

This manuscript is the first report that describes the effects of curcumin toward human osteoclastogenesis, and builds the framework for clinical trials of curcumin in the treatment of cancer-induced lytic bone disease.

PMID:
18766375
DOI:
10.1007/s00432-008-0461-8
[Indexed for MEDLINE]

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