Format

Send to

Choose Destination
Vet Clin Pathol. 2008 Sep;37(3):328-31. doi: 10.1111/j.1939-165X.2008.00055.x.

Cross-reactivity of anti-chicken IgY antibody with immunoglobulins of exotic avian species.

Author information

1
Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, Miami, FL 33101, USA. c.cray@miami.edu

Abstract

BACKGROUND:

A major challenge in the serologic diagnosis of infectious diseases in exotic birds is the limited availability of species-specific antibodies.

OBJECTIVES:

The purpose of the current study was to determine if there is cross reactivity between commercially available anti-chicken IgY antibodies and immunoglobulins of several avian species, with particular emphasis on psittacines.

METHODS:

To quantitate the reactivity with anti-chicken IgY, Western blot analysis was performed using plasma samples from many different avian species. Results were compared with gamma globulin fraction quantitation obtained by protein electrophoresis.

RESULTS:

By Western blot, 2 protein bands corresponding to the heavy and light chains of chicken IgY were identified in species from 21 avian orders using 1 of 2 rabbit anti-chicken IgY antibodies. Densitometric analysis showed that the amount of immunoglobulin estimated from Western blots correlated strongly with data from protein electrophoresis assays.

CONCLUSIONS:

The results demonstrate that some commercially available anti-chicken IgY antibodies exhibit good cross-reactivity with most avian species.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center