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J Neurosci Methods. 2008 Oct 30;175(1):88-95. doi: 10.1016/j.jneumeth.2008.08.004. Epub 2008 Aug 12.

Microinjection into cultured hippocampal neurons: a straightforward approach for controlled cellular delivery of nucleic acids, peptides and antibodies.

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1
Zentrum für Molekulare Neurobiologie Hamburg, ZMNH, Universität Hamburg, Hamburg, Germany.

Abstract

Functional studies in neurons often require controllable simultaneous delivery of different molecules to individual cells within networks. Microinjection represents a suitable and alternative method to deliver cDNAs, oligonucleotides, siRNAs, peptides or antibodies for expression, expression knockdown or loss-of-function studies, respectively. Moreover, molecules can be systematically applied to individual neurons in a controlled manner without affecting neighbouring cells. Establishment of microinjection is often complicated and time consuming. Here we describe a simple and reliable protocol for molecular cell biologists to establish injection of various molecules (ng to microg range) to living neurons in a reasonable period of time.

PMID:
18761372
DOI:
10.1016/j.jneumeth.2008.08.004
[Indexed for MEDLINE]

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