Abstract
Capillary electrophoresis (CE) with UV detection for the simultaneous and short-time analysis of clenbuterol, salbutamol, procaterol, fenoterol is described and validated. Optimized conditions were found to be a 10 mmoll(-1) borate buffer (pH 10.0), an separation voltage of 19 kV, and a separation temperature of 32 degrees C. Detection was set at 205 nm. Under the optimized conditions, analyses of the four analytes in pharmaceutical and human urine samples were carried out in approximately 1 min. The interference of the sample matrix was not observed. The LOD (limits of detection) defined at S/N of 3:1 was found between 0.5 and 2.0 mgl(-1) for the analytes. The linearity of the detector response was within the range from 2.0 to 30 mgl(-1) with correlation coefficient >0.996.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenergic beta-Agonists / analysis*
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Adrenergic beta-Agonists / chemistry
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Adrenergic beta-Agonists / isolation & purification
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Adrenergic beta-Agonists / urine*
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Albuterol / analysis
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Albuterol / chemistry
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Albuterol / isolation & purification
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Albuterol / urine
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Buffers
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Clenbuterol / analysis
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Clenbuterol / chemistry
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Clenbuterol / isolation & purification
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Clenbuterol / urine
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Electrophoresis, Capillary
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Fenoterol / analysis
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Fenoterol / chemistry
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Fenoterol / isolation & purification
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Fenoterol / urine
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Humans
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Hydrogen-Ion Concentration
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Linear Models
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Pharmaceutical Preparations / chemistry*
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Procaterol / analysis
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Procaterol / chemistry
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Procaterol / isolation & purification
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Procaterol / urine
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Reproducibility of Results
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Sensitivity and Specificity
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Temperature
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Time Factors
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Ultraviolet Rays
Substances
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Adrenergic beta-Agonists
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Buffers
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Pharmaceutical Preparations
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Fenoterol
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Albuterol
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Procaterol
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Clenbuterol