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J Virol Methods. 2008 Nov;153(2):250-2. doi: 10.1016/j.jviromet.2008.07.025. Epub 2008 Sep 19.

Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays.

Author information

1
Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan.

Abstract

A platform for assaying avian influenza H5N1 viruses that involves measuring the ac immunomagnetic reduction of a magnetic reagent mixed with a detected sample is developed. The magnetic reagent contained magnetic nanoparticles coated with antibodies. To achieve an ultra-high sensitivity assay, a system utilizing a high-transition-temperature superconducting quantum interference device was used to sense the immunomagnetic reduction of the reagents. The results confirmed the ultra-high sensitivity of the immunomagnetic reduction assay on H5N1.

PMID:
18760307
DOI:
10.1016/j.jviromet.2008.07.025
[Indexed for MEDLINE]

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