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Nat Methods. 2008 Oct;5(10):869-72. doi: 10.1038/nmeth.1250. Epub 2008 Aug 31.

Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans.

Author information

1
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, New York 10032, USA. md2398@columbia.edu

Abstract

We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately.

PMID:
18758453
PMCID:
PMC2693092
DOI:
10.1038/nmeth.1250
[Indexed for MEDLINE]
Free PMC Article

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