a, JUNQ compartment tightly co-localizes with nuclear membrane. DNA is visualized with DAPI (blue), nucleoplasm with NLS–TFP (red). GFP–Ubc9ts (top) at 37 °C and GFP–VHL (bottom) in cim3-1 cells. Two-dimensional projections of de-convoluted Z-series are shown. b, The JUNQ compartment, shown here for GFP–VHL, does not localize to the spindle pole body (marked by Spc42–CHFP), and is in close proximity to the endoplasmic reticulum visualized with Sec63–CHFP (c). d, The JUNQ compartment (upper panel, CHFP–VHL, red), but not the IPOD (Act1–E364K–CHFP, lower panel), concentrates 26S proteasomes (visualized with Cim5–GFP for regulatory particle and Pre6–GFP for core particle, green). e, Hsp104 localizes to both compartments. JUNQ and IPOD were formed by expressing CHFP–Ubc9ts (upper panel) or CHFP–VHL (lower panel). Note Hsp104 also accumulates in an IPOD structure (blue arrow) independently of ectopically-expressed aggregating protein when CHFP–VHL is expressed in cim3-1 cells at 30 °C (middle panel). f, The IPOD, shown here for GFP–Ubc9ts, co-localizes with CHFP–Atg8. Some CHFP–Atg8 can also be seen in the pre-autophagosomal structure (PAS).