Format

Send to

Choose Destination
Development. 2008 Oct;135(19):3239-46. doi: 10.1242/dev.022624. Epub 2008 Aug 28.

Dual roles of Incenp crucial to the assembly of the acentrosomal metaphase spindle in female meiosis.

Author information

1
Wellcome Trust Centre for Cell Biology, The University of Edinburgh, Edinburgh, UK.

Abstract

Spindle formation in female meiosis differs from mitosis in many animals, as it takes place independently of centrosomes, and the molecular requirements of this pathway remain to be understood. Here, we report two crucial roles of Incenp, an essential subunit of the chromosomal passenger complex (the Aurora B complex), in centrosome-independent spindle formation in Drosophila female meiosis. First, the initial assembly of spindle microtubules is drastically delayed in an incenp mutant. This clearly demonstrates, for the first time, a crucial role for Incenp in chromosome-driven spindle microtubule assembly in living oocytes. Additionally, Incenp is necessary to stabilise the equatorial region of the metaphase I spindle, in contrast to mitosis, where the equivalent function becomes prominent after anaphase onset. Our analysis suggests that Subito, a kinesin-6 protein, cooperates with Incenp for this latter function, but not in microtubule assembly. We propose that the two functions of Incenp are part of the mechanisms that compensate for the lack of centrosomes during meiotic spindle formation.

PMID:
18755775
PMCID:
PMC2536564
DOI:
10.1242/dev.022624
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center