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J Med Microbiol. 1991 Aug;35(2):107-10.

An improved selective medium for the isolation of Escherichia coli O157.

Author information

1
Public Health Laboratory, Northern General Hospital, Sheffield.

Abstract

Sorbitol-MacConkey medium has become widely used for the isolation of verotoxigenic (VT+) Escherichia coli O157. However, many organisms other than VT+ E. coli O157, especially other serogroups of E. coli and Proteus spp., may not ferment sorbitol, and thus may be confused initially with VT+ E. coli O157. Rhamnose is not fermented by VT+ E. coli O157, but is by most sorbitol non-fermenting E. coli of other serogroups. Cefixime is a cephalosporin antibiotic that is more active against Proteus spp. than against E. coli. Inclusion of rhamnose and cefixime in sorbitol-MacConkey agar improves its selectivity for the isolation of VT+ E. coli O157.

PMID:
1875390
DOI:
10.1099/00222615-35-2-107
[Indexed for MEDLINE]

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