Send to

Choose Destination
Curr Protoc Stem Cell Biol. 2008 Aug;Chapter 2:Unit 2A.8.1-2A.8.14. doi: 10.1002/9780470151808.sc02a08s6.

Isolation and visualization of mouse placental hematopoietic stem cells.

Author information

University of California Los Angeles, Los Angeles, California, USA.


This unit describes the isolation of hematopoietic stem cells (HSCs) from the mouse placenta. The placenta was recently identified as an important hematopoietic site that generates HSCs de novo and provides a transitory niche for a large pool of HSCs during midgestation. This protocol includes a dissection technique for murine placenta, the mechanical and enzymatic steps of placental tissue dissociation, and phenotypical identification and isolation of HSCs. It also contains a method for immunohistochemical analysis of placenta tissue sections to visualize developing HSCs in the placenta.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center