Format

Send to

Choose Destination
See comment in PubMed Commons below
Mol Biol Cell. 2008 Nov;19(11):4730-7. doi: 10.1091/mbc.E08-06-0665. Epub 2008 Aug 20.

CLASP modulates microtubule-cortex interaction during self-organization of acentrosomal microtubules.

Author information

1
The University of British Columbia, Vancouver, BC V6T 1Z4, Canada.

Abstract

CLASP proteins associate with either the plus ends or sidewalls of microtubules depending on the subcellular location and cell type. In plant cells, CLASP's distribution along the full length of microtubules corresponds with the uniform anchorage of microtubules to the cell cortex. Using live cell imaging, we show here that loss of CLASP in Arabidopsis thaliana results in partial detachment of microtubules from the cortex. The detached portions undergo extensive waving, distortion, and changes in orientation, particularly when exposed to the forces of cytoplasmic streaming. These deviations from the normal linear polymerization trajectories increase the likelihood of intermicrotubule encounters that are favorable for subsequent bundle formation. Consistent with this, cortical microtubules in clasp-1 leaf epidermal cells are hyper-parallel. On the basis of these data, we identify a novel mechanism where modulation of CLASP activity governs microtubule-cortex attachment, thereby contributing to self-organization of cortical microtubules.

PMID:
18716054
PMCID:
PMC2575154
DOI:
10.1091/mbc.E08-06-0665
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center