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Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12469-73. doi: 10.1073/pnas.0805012105. Epub 2008 Aug 18.

Normal ovarian surface epithelial label-retaining cells exhibit stem/progenitor cell characteristics.

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1
Pediatric Surgical Research Laboratories, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.

Erratum in

  • Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):19024.

Abstract

Ovulation induces cyclic rupture and regenerative repair of the ovarian coelomic epithelium. This process of repeated disruption and repair accompanied by complex remodeling typifies a somatic stem/progenitor cell-mediated process. Using BrdU incorporation and doxycycline inducible histone2B-green fluorescent protein pulse-chase techniques, we identify a label-retaining cell population in the coelomic epithelium of the adult mouse ovary as candidate somatic stem/progenitor cells. The identified population exhibits quiescence with asymmetric label retention, functional response to estrous cycling in vivo by proliferation, enhanced growth characteristics by in vitro colony formation, and cytoprotective mechanisms by enrichment for the side population. Together, these characteristics identify the label-retaining cell population as a candidate for the putative somatic stem/progenitor cells of the coelomic epithelium of the mouse ovary.

PMID:
18711140
PMCID:
PMC2527935
DOI:
10.1073/pnas.0805012105
[Indexed for MEDLINE]
Free PMC Article
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