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J Bacteriol. 2008 Oct;190(20):6922-6. doi: 10.1128/JB.00934-08. Epub 2008 Aug 15.

Interaction of penicillin-binding protein 2 with soluble lytic transglycosylase B1 in Pseudomonas aeruginosa.

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1
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

Abstract

Soluble lytic transglycosylase B1 from Pseudomonas aeruginosa was coupled to Sepharose and used to immobilize interaction partners from membrane protein extracts. Penicillin-binding protein 2 (PBP2) was identified as a binding partner, suggesting that the two proteins function together in the biosynthesis of peptidoglycan. By use of an engineered truncated derivative, the N-terminal module of PBP2 was found to confer the binding properties.

PMID:
18708507
PMCID:
PMC2566182
DOI:
10.1128/JB.00934-08
[Indexed for MEDLINE]
Free PMC Article
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