Send to

Choose Destination
Int J Biochem Cell Biol. 2009 Mar;41(3):625-31. doi: 10.1016/j.biocel.2008.07.005. Epub 2008 Jul 25.

The dynamics of the nitric oxide release-transient from stretched muscle cells.

Author information

Department of Human Anatomy and Cell Science, Faculty of Medicine, The University of Manitoba, Canada.


Potent nitric oxide (NO) signals are described for many forms of cell-cell communication. Although NO plays a significant role in skeletal muscle metabolism and contractility and in precursor activation during muscle formation and stretching, there is no direct evidence of stretch-induced NO release from muscle. Differentiated muscle cell cultures from normal and dystrophic mdx mice were preloaded with the NO-specific dye DAF-2 (diaminofluorescein-2) before stretching. NO release was detected by video-microscopy. NO was released rapidly from wild-type (WT) cells after stretch and intensity declined rapidly to a plateau. Mdx cells showed much less NO release. Direct observations of the time-course of stretch-induced NO release in WT cells is congruent with the hypothesis of NO-mediated stretch activation of satellite cells in normal skeletal muscle. Distinct differences in the time-course between normal and dystrophic cells indicate visualization methods for NO release will be a sensitive measure of NOS-1 restoration following diverse treatment approaches to muscular dystrophy.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center