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J Virol Methods. 2008 Nov;153(2):276-9. doi: 10.1016/j.jviromet.2008.07.001. Epub 2008 Aug 23.

Using G-deleted vesicular stomatitis virus to probe the innate anti-viral response.

Author information

1
Department of Medical Sciences, Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, 1200 Main Street West, MDCL 5023, Hamilton, Ontario, Canada L8N 3Z5.

Abstract

A method is described for the use of a G-deleted, conditionally replicating version of vesicular stomatitis virus (VSV) to measure alterations to the innate anti-viral state of cells in vitro. By co-transfecting a gene of interest with an expression vector for VSV-G one can directly measure the replication of the virus in the transfected cells as non-transfected cells will fail to produce infectious progeny due to the lack of glycoprotein in these non-transfected cells. This allows the investigator to focus exclusively on the anti-viral state induced or inhibited in the transfected cells allowing for screening and quantitative analysis of viral or cellular genes that may modify the anti-viral state.

PMID:
18692525
DOI:
10.1016/j.jviromet.2008.07.001
[Indexed for MEDLINE]

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