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Cell. 2008 Aug 8;134(3):534-45. doi: 10.1016/j.cell.2008.07.009.

A protein domain-based interactome network for C. elegans early embryogenesis.

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1
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. mboxem@partners.org

Erratum in

  • Cell. 2012 Dec 21;151(7):1633.

Abstract

Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.

PMID:
18692475
PMCID:
PMC2596478
DOI:
10.1016/j.cell.2008.07.009
[Indexed for MEDLINE]
Free PMC Article

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