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Arthritis Res Ther. 2008;10(4):R89. doi: 10.1186/ar2473. Epub 2008 Aug 11.

Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix.

Author information

1
Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232, USA. hazel.tapp@carolinashealthcare.org

Abstract

INTRODUCTION:

Adult mesenchymal stem cell therapy has a potential application in the biological treatment of disc degeneration. Our objectives were: to direct adipose-derived mesenchymal stem cells (AD-MSC) from the sand rat to produce a proteoglycan and collagen type I extracellular matrix (ECM) rich in known ECM components of the annulus fibrosis of disc; and to stimulate proteoglycan production by co-culture of human annulus cells with AD-MSC.

METHODS:

AD-MSC were isolated and characterised by adherence to plastic, appropriate expression of cluster of differentiation (CD) markers, and differentiation to osteoblasts and chondrocytes in vitro. AD-MSC were grown in three-dimensional (3D) culture and treated with or without transforming growth factor beta (TGFbeta) to direct them to produce annulus-like ECM as determined by proteoglycan content and collagen expression. AD-MSC were co-cultured with human annulus cells and grown in 3D culture.

RESULTS:

AD-MSC produced a proteoglycan and collagen type I rich ECM after treatment with TGFbeta in 3D culture as confirmed by a 48% increase in proteoglycan content assayed by 1,9-dimethylmethylene blue (DMB), and by immunohistochemical identification of ECM components. Co-culture of human annulus and sand rat AD-MSC in 3D culture resulted in a 20% increase in proteoglycan production compared with the predicted value of the sum of the individual cultures.

CONCLUSION:

Results support the hypothesis that AD-MSC have potential in cell-based therapy for disc degeneration.

PMID:
18691412
PMCID:
PMC2575603
DOI:
10.1186/ar2473
[Indexed for MEDLINE]
Free PMC Article
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