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Invest Ophthalmol Vis Sci. 2009 Jan;50(1):214-23. doi: 10.1167/iovs.08-2302. Epub 2008 Aug 8.

Detection of optic nerve head neural canal opening within histomorphometric and spectral domain optical coherence tomography data sets.

Author information

1
Optic Nerve Head Research Laboratory, Devers Eye Institute, Legacy Health System, Portland, OR 97208-3950, USA.

Abstract

PURPOSE:

To assess the ability to detect the neural canal opening (NCO) and its characteristics within three-dimensional (3-D) histomorphometric and 3-D spectral domain optical coherence tomography (SD-OCT) reconstructions of the optic nerve head from nonhuman primate (NHP) eyes.

METHODS:

NCO was delineated within 40 radial, sagittal sections of 3-D histomorphometric reconstructions of 44 normal eyes of 38 NHPs, each perfusion fixed at IOP 10 mm Hg, and 3-D SD-OCT (Spectralis; Heidelberg Engineering, Heidelberg, Germany) volumes acquired in vivo from a separate group of 33 normal eyes of 24 NHPs. Within all reconstructions, a least-squares ellipse was fitted to the 80 NCO points. For each eye, the dimensions and plane error (a gauge of planarity) of the fitted ellipse were calculated.

RESULTS:

The NCO was successfully delineated within every section of each histomorphometric and SD-OCT reconstruction. Median plane error was similar within histomorphometric and SD-OCT volumes (8 microm, range 4-19, histomorphometry, and 10 microm, range 4-26, SD-OCT) and was small relative to the size of the ellipse. Median histomorphometric ellipse dimensions were 1453 mum (major axis, range 1218-1737) and 1066 microm (minor axis, range 808-1263). Median SD-OCT ellipse dimensions were 1512 microm (major axis, range 1191-1865) and 1060 microm (minor axis, range 772-1248).

CONCLUSIONS:

The NCO is biologically continuous and relatively planar within all 3-D histomorphometric and SD-OCT reconstructions. These characteristics support its further evaluation as a reference plane for cross-sectional and longitudinal measurement of optic nerve head structures using 3-D SD-OCT.

PMID:
18689697
PMCID:
PMC2726821
DOI:
10.1167/iovs.08-2302
[Indexed for MEDLINE]
Free PMC Article

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