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Int J Cancer. 2008 Nov 1;123(9):2105-12. doi: 10.1002/ijc.23685.

Multiple pathways for Epstein-Barr virus episome loss from nasopharyngeal carcinoma.

Author information

1
Lineberger Comprehensive Cancer Center, Center for AIDS Research and Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC, USA. ddittmer@med.unc.edu

Abstract

Epstein-Barr virus (EBV) is the prototypical example for episomal persistence of genetic information. Yet, little is known about how this viral episome is lost. Episome loss occurs naturally in nasopharyngeal carcinoma (NPC) upon explantation into culture. Using whole-genome profiling, we found evidence for 2 different pathways of episome loss: (i) rapid loss of the entire episome or (ii) successive mutation/deletion of the episome until at least 1 essential cis-element is destroyed. This second phenotype was seen in a clone of HONE-1 NPC cells that maintains the EBV episome for prolonged time in culture. The conceptual insights provided by our quantitative analysis should aid our understanding of mammalian episomes, as well as lead to designs to cure latent viral infection.

PMID:
18688856
PMCID:
PMC2866115
DOI:
10.1002/ijc.23685
[Indexed for MEDLINE]
Free PMC Article

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