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Zhonghua Yan Ke Za Zhi. 2008 Feb;44(2):122-7.

[Experimental research of small-incision Descemet's stripping endothelial keratoplasty].

[Article in Chinese]

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The Eye Center, Third Hospital, Peking University, Beijing 100083, China.



To investigate the surgical procedure, clinical efficacy, complications, density of endothelial cells and histological changes after Descemet's stripping endothelial keratoplasty (DSEK) surgery.


It was a experimental study. Twenty four New Zealand rabbits were divided into 3 groups, 8 rabbits per group. Donor grafts were dissected from 16 New Zealand rabbit eyes. Group A was experimental group, a 5 mm limbal tunnel incision was made. Descemet's membrane was striped off at 10 mm diameter, then the same diameter donor cornea (including Descemet's membrane and endothelium with a little of posterior stroma) was inserted into the recipient's anterior chamber. Air was injected into the anterior chamber to press the graft up against the recipient cornea. Group B was the control group, only striped the Descemet's membrane at the recipient cornea. Group C was the experiment control group, the procedure was similar to the group A, but the donor graft was without endothelial cells.


All corneas of group A were transparent, and the mean density of the endothelial cells was (2195 +/- 77)/mm2 (t = 12.455, P < 0.001). Endothelial grafts attached to the recipients well and no scar formation between them under histological observation. The corneas were severe edema in groups B and C one month after surgery.


DSEK is a safe surgery, can be recovered rapidly with little damages, and without interface scar formation after surgery. DSEK may be the first choice for the treatment of bullous keratopathy.

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