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Anal Biochem. 2008 Dec 15;383(2):320-2. doi: 10.1016/j.ab.2008.07.020. Epub 2008 Jul 26.

Microplate quantification of enzymes of the plant ascorbate-glutathione cycle.

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Université d'Avignon et des Pays de Vaucluse, Laboratoire Physiologie des Fruits et Légumes, F-84018 Avignon, France.


Here, we describe microplate assays for determining the specific activities of four enzymes that constitute the ascorbate-glutathione cycle: APX, MDHAR, DHAR, and GR. In plants, these enzymes play a major role in detoxifying reactive oxygen species produced in cells under environmental stress. This work presents the development of plate reader assays to allow rapid analysis of the ascorbate-glutathione cycle activity using tomato fruits subjected to salt stress as a model. With this method, it is possible to analyze easily in one day the activities of the four enzymes for 30 experimental samples, all in triplicate and with blanks.

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