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Plant Mol Biol. 1991 Apr;16(4):593-600.

Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina.

Author information

1
Institut für Biologie II, Lehrstuhl für Zellbiologie, Freiburg, Germany.

Abstract

Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence of Hoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated from restriction enzyme analysis. A physical map for six restriction enzymes (Bam HI, Bge I, Eco RI, Pst I, Sac I and Sal I) has been constructed. Genes coding for the small subunit of rRNA, cytochrome oxidase subunits I and II, and apocytochrome b were localized on this map using Southern blot hybridization with heterologous gene probes from Oenothera. Genes for 5S rRNA and NADH dehydrogenase subunit 5 are absent from P. salina mtDNA. The mitochondrial genome, being the first analysed to this extent in chromophytic algae, should be valuable for taxonomic and phylogenetic studies.

PMID:
1868198
DOI:
10.1007/bf00023424
[Indexed for MEDLINE]

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