Send to

Choose Destination
Plant Mol Biol. 1991 Apr;16(4):593-600.

Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina.

Author information

Institut für Biologie II, Lehrstuhl für Zellbiologie, Freiburg, Germany.


Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence of Hoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated from restriction enzyme analysis. A physical map for six restriction enzymes (Bam HI, Bge I, Eco RI, Pst I, Sac I and Sal I) has been constructed. Genes coding for the small subunit of rRNA, cytochrome oxidase subunits I and II, and apocytochrome b were localized on this map using Southern blot hybridization with heterologous gene probes from Oenothera. Genes for 5S rRNA and NADH dehydrogenase subunit 5 are absent from P. salina mtDNA. The mitochondrial genome, being the first analysed to this extent in chromophytic algae, should be valuable for taxonomic and phylogenetic studies.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center