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Opt Express. 2008 Aug 4;16(16):12227-38.

Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology.

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  • 1Hamamatsu Photonics K. K., 5000 Hirakuchi, Hamamatsu-City, Shizuoka-Pref. 434-8601, Japan.


We have developed a Linnik-type interference microscope provided with a low-coherent light source to obtain topographic images of an intact cellular membrane on a nanometer scale. Our technique is based on measurement of the interference between light reflected from the cell surface and a reference beam. The results show full field surface topography of cultured cells and reveal an intrinsic membrane motion of tens of nanometers.

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