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J Pharm Biomed Anal. 2008 Sep 10;48(1):100-4. doi: 10.1016/j.jpba.2008.05.027. Epub 2008 May 28.

Chemical fingerprint and quantitative analysis of Salvia plebeia R.Br. by high-performance liquid chromatography.

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1
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.

Abstract

To control the quality of Salvia plebeia R.Br., a simple and reliable method of high-performance liquid chromatography coupled with photodiode array detector (HPLC-DAD) was developed both for fingerprint analysis and quantitative determination of seven bioactive compounds, namely caffeic acid, luteolin-7-glucoside, nepetin-7-glucoside, homoplantaginin, luteolin, nepetin and hispidulin. In fingerprint analysis, twelve peaks were selected as characteristic peaks. In quantitative analysis, seven compounds showed good regression (R2>0.9995) within test ranges and the recovery of the method was in the range of 91.7-103.2%. The content ranges (mg/g) of seven compounds in the collected samples of S. plebeia were 0.80-1.67 (hispidulin), 2.18-5.75 (homoplantaginin), 0.52-1.22 (nepetin), 1.56-3.48 (nepetin-7-glucoside), 0.12-0.24 (luteolin), 0.97-2.22 (luteolin-7-glucoside) and 0.21-0.44 (caffeic acid), respectively. From the results obtained, the content of homoplantaginin was the highest. In addition, luteolin and luteolin-7-glucoside were isolated for the first time from S. plebeia.

PMID:
18678457
DOI:
10.1016/j.jpba.2008.05.027
[Indexed for MEDLINE]

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