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Biochem Biophys Res Commun. 2008 Oct 10;375(1):33-7. doi: 10.1016/j.bbrc.2008.07.113. Epub 2008 Jul 31.

Identification and characterisation of a nuclear localisation signal in the SMN associated protein, Gemin4.

Author information

1
Department of Veterinary Pathobiology, Bond Life Sciences Center, 1201 Rollins Road, University of Missouri, Columbia, MO 65211, USA.

Abstract

Gemin4 is a ubiquitously expressed multifunctional protein that is involved in U snRNP assembly, apoptosis, nuclear/cytoplasmic transportation, transcription, and RNAi pathways. Gemin4 is one of the core components of the Gemin-complex, which also contains survival motor neuron (SMN), the seven Gemin proteins (Gemin2-8), and Unrip. Mutations in the SMN1 gene cause the autosomal recessive disorder spinal muscular atrophy (SMA). Although the functions assigned to Gemin4 predominantly occur in the nucleus, the mechanisms that mediate the nuclear import of Gemin4 remain unclear. Here, using a novel panel of Gemin4 constructs we identify a canonical nuclear import sequence (NLS) in the N-terminus of Gemin4. The Gemin4 NLS is necessary and independently sufficient to mediate nuclear import of Gemin4. This is the first functional NLS identified within the SMN-Gemin complex.

PMID:
18675250
PMCID:
PMC3613997
DOI:
10.1016/j.bbrc.2008.07.113
[Indexed for MEDLINE]
Free PMC Article

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