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J Clin Virol. 2008 Sep;43(1):96-101. doi: 10.1016/j.jcv.2008.05.005. Epub 2008 Jul 31.

One-step RT-PCR for detection of Zika virus.

Author information

1
Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal.

Abstract

BACKGROUND:

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive.

OBJECTIVE:

To develop a one-step RT-PCR assay to detect ZIKV in human serum.

STUDY DESIGN:

An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts.

RESULTS:

The assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected.

CONCLUSIONS:

The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.

PMID:
18674965
DOI:
10.1016/j.jcv.2008.05.005
[Indexed for MEDLINE]

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