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J Proteome Res. 2008 Sep;7(9):4022-30. doi: 10.1021/pr800301h. Epub 2008 Jul 29.

Optimizing sample handling for urinary proteomics.

Author information

1
Department of Urology, Urological Diseases Research Center, Harvard Medical School and Children's Hospital Boston, Boston, Massachusetts 02115, USA. richard.lee@childrens.harvard.edu

Abstract

Interrogation of the urinary proteome for clinically useful biomarkers of disease will require normalization of methods for protein extraction and sample handling. Variations in collection methods and other procedures may introduce significant discrepancies in qualitative and quantitative measurements. Here we demonstrate that the method of protein extraction, length of handling at room temperature, and repetitive freeze-thaw cycles do not seem to alter the urinary proteome at either the protein or peptide level in a manner that degrades information obtainable by mass spectrometry.

PMID:
18662026
PMCID:
PMC5451898
DOI:
10.1021/pr800301h
[Indexed for MEDLINE]
Free PMC Article

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