Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release

Biomaterials. 2008 Oct;29(30):4039-44. doi: 10.1016/j.biomaterials.2008.07.005. Epub 2008 Jul 26.

Abstract

The ability of nitric oxide (NO)-releasing xerogels to reduce fibrinogen-mediated adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli is described. A negative correlation was observed between NO surface flux and bacterial adhesion for each species tested. For S. aureus and E. coli, reduced adhesion correlated directly with NO flux from 0 to 30 pmol cm(-2)s(-1). A similar dependence for S. epidermidis was evident from 18 to 30 pmol cm(-2)s(-1). At a NO flux of 30 pmol cm(-2)s(-1), surface coverage of S. aureus, S. epidermidis, and E. coli was reduced by 96, 48, and 88%, respectively, compared to non-NO-releasing controls. Polymeric NO release was thus demonstrated to be an effective approach for significantly reducing fibrinogen-mediated adhesion of both gram-positive and gram-negative bacteria in vitro, thereby illustrating the advantage of active NO release as a strategy for inhibiting bacterial adhesion in the presence of pre-adsorbed protein.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Bacterial Adhesion / drug effects*
  • Coated Materials, Biocompatible / administration & dosage*
  • Coated Materials, Biocompatible / chemistry*
  • Dose-Response Relationship, Drug
  • Escherichia coli / physiology
  • Fibrinogen / administration & dosage*
  • Fibrinogen / chemistry*
  • Materials Testing
  • Nitric Oxide / administration & dosage*
  • Nitric Oxide / chemistry*
  • Protein Binding
  • Staphylococcus aureus / physiology
  • Staphylococcus epidermidis / physiology

Substances

  • Coated Materials, Biocompatible
  • Nitric Oxide
  • Fibrinogen