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Virology. 2008 Sep 15;379(1):10-9. doi: 10.1016/j.virol.2008.06.023. Epub 2008 Jul 25.

Biochemical and structural characterisation of membrane-containing icosahedral dsDNA bacteriophages infecting thermophilic Thermus thermophilus.

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1
Department of Biological and Environmental Sciences and Institute of Biotechnology, Biocenter 2, FIN-00014, University of Helsinki, Finland.

Erratum in

  • Virology. 2012 Jun 5;427(2):224.

Abstract

Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of these viruses, growing to a high titer, appeared very similar but not identical. The most stable virus, P23-77, was chosen for more detailed studies. Analysis of highly purified P23-77 by thin layer chromatography for neutral lipids showed lipid association with the virion. Cryo-EM based three-dimensional image reconstruction of P23-77 to 1.4 nm resolution revealed an icosahedrally-ordered protein coat, with spikes on the vertices, and an internal membrane. The capsid architecture of P23-77 is most similar to that of the archaeal virus SH1. These findings further complicate the grouping of icosahedrally-symmetric viruses containing an inner membrane. We propose a single superfamily or order with members in several viral families.

PMID:
18657283
DOI:
10.1016/j.virol.2008.06.023
[Indexed for MEDLINE]
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