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Proteomics. 2008 Jul;8(14):2907-10. doi: 10.1002/pmic.200800083.

Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins.

Author information

1
Discovery Research Laboratory, Tokyo R&D Center, Daiichi Pharmaceutical Co., Ltd., Daiichi-Sankyo group, Tokyo, Japan.

Abstract

RNF43 is an oncogenic RING finger protein overexpressed in colorectal cancer. To dissect its biological functions, we explored RNF43-interacting proteins by pull-down assay and MS. We identified a heterodimer, p54nrb and PSF, as RNF43's binding partners and confirmed their physical interaction in vivo by the co-immunoprecipitation experiment. Immunofluorescence analysis revealed that co-expression of PSF relocates RNF43 from the nuclear periphery to the nucleoplasm. Thus, proteomic identification of RNF43-associated proteins sheds light on its dynamic interaction network in nuclear events.

PMID:
18655028
DOI:
10.1002/pmic.200800083
[Indexed for MEDLINE]
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