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Zhongguo Zhong Yao Za Zhi. 2008 May;33(9):1060-3.

[Changes of proteomic spectra of total flavones of Hippophae rhamnoides on myocardial protection].

[Article in Chinese]

Author information

1
Department of Cardiovascular Research, Baotou Medical college, Baotou 014010, China. lzr13039570839@sina.com

Abstract

OBJECTIVE:

To study the mechanisms of the anti-myocardial ischemia of total flavones of Hippophae rhamnoides (TFH) at the level of proteome.

METHOD:

Surface enhanced laser desorption/ionization (SELDI) mass spectrometry with protein chip IMAC3, SAX2 and NP20 was performed to compare the differentially expressed protein in myocardial ischemia in the TFH-treated groups with the 0.9% sodium chloride groups. Protein chips were examined in PBS II - C protein chip reader (ciphergen ciosystem inc) and the protein profiling was analyzed by Proteinchip Software 3. 0. 2.

RESULT:

The revealed six peaks had significant difference between the TFH-treated groups and the control groups, one of which were up-regulated in the TFH-treated groups, and the other were down-regulated. And in these six distinct proteins, there were four proteins on the IMAC3 chips and one protein on the SAX2 chips.

CONCLUSION:

The TFH could prevent the myocardium from ischemia via regulating expression of different proteins.

PMID:
18652358
[Indexed for MEDLINE]

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