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Wei Sheng Wu Xue Bao. 2008 May;48(5):690-4.

[Apoptosis of HL-60 cells induced by crystal proteins from Bacillus thuringiensis Bt9875].

[Article in Chinese]

Author information

1
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Abstract

OBJECTIVE:

To study the effect of Bt9875 crystal protein treated with proteinase K on human cancer cells, HL-60.

METHODS:

We used the methods of Thiazolyl Blue Tetrazolium Bromide, fluorescence microscopy examination, agarose gel electrophores and flow cytometry to detect the growth inhibition rate and apoptosis characters of the HL-60 cells that were treated with different concentration of Bt9875 crystal protein.

RESULTS:

Bt9875 crystal protein inhibited the growth of HL-60 cells evidently in a dose-dependent manner, with minimal effects on normal human peripheral blood mononuclear cells (PBMCs). The nuclei of HL-60 cells showed the characteristics of apoptosis. The analysis by flow cytometry indicated that the apoptosis rate of HL-60 cells was 52% after treatment with Bt9875 crystal protein (100 microg/mL). DNA analyzed by agarose gel electrophoresis showed "ladder" pattern.

CONCLUSION:

Bt9875 crystal protein could inhibit the growth of HL-60 and induced its apoptosis, which provided a foundation for use of Bt9875 crystal protein to cure the acute myeloid leukemia.

PMID:
18652305
[Indexed for MEDLINE]

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