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Proteomics. 2008 Aug;8(16):3371-81. doi: 10.1002/pmic.200800111.

Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery.

Author information

1
Department of Biochemistry, Yonsei Proteome Research Center and Biomedical Proteome Research Center, Yonsei University, Seoul, Korea.

Abstract

A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.

PMID:
18651672
DOI:
10.1002/pmic.200800111
[Indexed for MEDLINE]

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