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Histochem Cell Biol. 2008 Sep;130(3):473-80. doi: 10.1007/s00418-008-0481-0. Epub 2008 Jul 22.

Quality control in molecular immunohistochemistry.

Author information

1
Department of Pathology, University of Washington, 1959 NE Pacific St, Box 35-6100, Seattle, WA, USA. ltrue@u.washington.edu

Abstract

Immunoperoxidase histochemistry is a widespread method of assessing expression of biomolecules in tissue samples. Accurate assessment of the expression levels of genes is critical for the management of disease, particularly as therapy targeted to specific molecules becomes more widespread. Determining the quality of preservation of macromolecules in tissue is important to avoid false negative and false positive results. In this review we discuss (1) issues of sensitivity (false negativity) and specificity (false positivity) of immunohistochemical stains, (2) approaches to better understanding differences in immunostains done by different laboratories (including the recently proposed MISFISHIE specification for tissue localization studies), and (3) approaches to assessing the quality of preservation of macromolecules in tissue, particularly in small biopsy samples.

PMID:
18648842
PMCID:
PMC2522330
DOI:
10.1007/s00418-008-0481-0
[Indexed for MEDLINE]
Free PMC Article
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