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Methods Mol Biol. 2008;420:265-75. doi: 10.1007/978-1-59745-583-1_16.

Imaging Drosophila pupal wing morphogenesis.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

Abstract

Drosophila pupal (P) wing development entails a series of dynamic developmental events, such as epithelial and glial morphogenesis, that are of outstanding interest to cell biologists. Here, we first describe how to prepare P and prepupal (PP) wings for immunofluorescence microscopy. This protocol has been optimized to visualize wing epithelial architecture, such as polarized cortical domains of planar cell polarity proteins. We then provide a protocol to prepare pupae for whole mount live imaging of P wings. This procedure has allowed us to live-image glial cell migration and proliferation along wing sensory nerves.

PMID:
18641953
DOI:
10.1007/978-1-59745-583-1_16
[Indexed for MEDLINE]

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