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Methods Mol Biol. 2008;420:253-63. doi: 10.1007/978-1-59745-583-1_15.

Immunolabeling of imaginal discs.

Author information

1
Institute for Genetics, University of Cologne, Cologne, Germany.

Abstract

Drosophila melanogaster imaginal discs are a widely used model system to study signal transduction, developmental, and cell biological processes. An important tool to study these processes is immunolabeling. Fluorochrome-conjugated antibody staining allows the researcher to determine the expression pattern and subcellular localization of several proteins in one imaginal disc. In this chapter, I will describe how to dissect, fix, and immunolabel imaginal discs. The protocol is streamlined so that the whole procedure can be performed in 1 d, including image acquisition.

PMID:
18641952
DOI:
10.1007/978-1-59745-583-1_15
[Indexed for MEDLINE]

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