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Diagn Microbiol Infect Dis. 2008 Oct;62(2):230-3. doi: 10.1016/j.diagmicrobio.2008.05.014. Epub 2008 Jul 21.

First molecular method for discriminating between Candida africana, Candida albicans, and Candida dubliniensis by using hwp1 gene.

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Department of Microbiological, Genetic and Molecular Sciences University of Messina, Salita Sperone, 31 98166 Messina, Italy.


The authors report here a polymerase chain reaction-based assay using a single primer pair (CR-f/CR-r), allowing discrimination between Candida africana and Candida albicans by using the hwp1 gene. The method also identifies Candida dubliniensis because it produces 3 different DNA fragments: approximately 700 bp for C. africana, 941 bp for C. albicans, and 569 for C. dubliniensis.

[Indexed for MEDLINE]

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