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J Proteomics. 2008 Aug 21;71(3):346-56. doi: 10.1016/j.jprot.2008.07.003. Epub 2008 Jul 12.

Protein identification pipeline for the homology-driven proteomics.

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1
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

Abstract

Homology-driven proteomics is a major tool to characterize proteomes of organisms with unsequenced genomes. This paper addresses practical aspects of automated homology-driven protein identifications by LC-MS/MS on a hybrid LTQ Orbitrap mass spectrometer. All essential software elements supporting the presented pipeline are either hosted at the publicly accessible web server, or are available for free download.

PMID:
18639657
PMCID:
PMC2644553
DOI:
10.1016/j.jprot.2008.07.003
[Indexed for MEDLINE]
Free PMC Article
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