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J Pharm Biomed Anal. 2008 Nov 4;48(3):965-8. doi: 10.1016/j.jpba.2008.05.039. Epub 2008 Jun 14.

SPE-HPTLC of procyanidins from the barks of different species and clones of Salix.

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Department of Pharmacognosy, Medical University of Gdańsk, Gen. J. Hallera 107 str., 80-416 Gdańsk, Poland.


A SPE-HPTLC method was developed for the qualitative and quantitative analysis of procyanidin B(1) in willow barks. The chromatography was performed on HPTLC silica gel layer with the mobile phase chloroform-ethanol-formic acid (50:40:6 v/v/v), in the Automatic Developing Chamber-ADC 2. The methanol extracts from willow barks were purified by SPE method on RP-18 silica gel columns with methanol-water (7:93 v/v) as the eluent. The presence of procyanidin B(1) was revealed in the majority of investigated willow barks. The content of procyanidin B(1) varied from 0.26 mg/g in the extract of Salix purpurea clone 1067-2.24 mg/g in the extract of Salix alba clone 1100. The method was validated for linearity, precision, LOD, LOQ and repeatability.

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